6PBV
Crystal structure of Fab668 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.065, 60.127, 76.338 |
| Unit cell angles | 72.43, 68.07, 83.58 |
Refinement procedure
| Resolution | 35.051 - 1.566 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.566 | 1.566 |
| Rpim | 0.072 | 0.389 |
| Number of reflections | 111126 | 5151 |
| <I/σ(I)> | 23.3 | 2.2 |
| Completeness [%] | 86.8 | |
| Redundancy | 7 | |
| CC(1/2) | 0.907 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M HEPES (pH 6.96) 8% Ethylene Glycol 16% PEG10000 |
