6PBA
Structure of ClpC1-NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.530, 59.120, 58.790 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 35.400 - 1.770 |
| R-factor | 0.19677 |
| Rwork | 0.195 |
| R-free | 0.22294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cn8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.081 | 0.513 |
| Rpim | 0.069 | |
| Number of reflections | 13940 | 780 |
| <I/σ(I)> | 41.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.1 M MES, pH 6.0, 40% PEG400, 5% PEG3000 |
