6PB3
Structure of Rhizobiales Trip13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 6 |
Unit cell lengths | 100.436, 100.436, 48.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 86.980 - 2.048 |
R-factor | 0.2106 |
Rwork | 0.208 |
R-free | 0.25170 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.829 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.980 | 2.100 |
High resolution limit [Å] | 2.048 | 2.050 |
Rmerge | 0.058 | 1.955 |
Rmeas | 0.061 | 2.057 |
Rpim | 0.019 | 0.635 |
Number of reflections | 17737 | 1347 |
<I/σ(I)> | 17.3 | 0.9 |
Completeness [%] | 99.3 | 99 |
Redundancy | 10.2 | 10.2 |
CC(1/2) | 0.999 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM sodium citrate pH 6.0, 1.6 M NH2SO4, and 0.2 M sodium/potassium tartrate |