6PAO
Structure of a bacterial Atm1-family ABC exporter with ATP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033202 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.164, 135.415, 191.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.103 - 3.650 |
| R-factor | 0.2531 |
| Rwork | 0.252 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pan |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.103 | 4.210 | 3.780 |
| High resolution limit [Å] | 3.650 | 3.900 | 3.650 |
| Rmerge | 0.076 | 1.852 | 2.002 |
| Rmeas | 0.080 | 1.945 | 2.146 |
| Rpim | 0.027 | 0.590 | 0.758 |
| Number of reflections | 27912 | 4675 | 2712 |
| <I/σ(I)> | 14.33 | 1.6 | 1.11 |
| Completeness [%] | 97.8 | 99.9 | 97.54 |
| Redundancy | 8.8 | 10.6 | 8.1 |
| CC(1/2) | 1.000 | 0.586 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 296 | NaCl, Tris, PEG2000MME, ATP, NDSB |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 296 | NaCl, Tris, PEG2000MME, ATP, NDSB |
