6PAN

Structure of a bacterial Atm1-family ABC exporter with ATP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97938
Spacegroup name	P 21 21 21
Unit cell lengths	95.500, 134.580, 190.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.708 - 3.400
R-factor	0.1933
Rwork	0.191
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hyd
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.710	3.522
High resolution limit [Å]	3.400	3.400
Rmerge	0.148	3.919
Rmeas	0.151	4.000
Rpim	0.029	0.792
Number of reflections	34343	3016
<I/σ(I)>	17.26	1.19
Completeness [%]	98.6	89.27
Redundancy	26.7	25.1
CC(1/2)	1.000	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	296	NaCl, Tris, PEG2000MME, ATP