6PAL
Bacteroides uniformis endo-laminarinase BuGH158 from the beta(1,3)-glucan utilization locus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.675, 106.030, 126.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.726 - 1.818 |
Rwork | 0.169 |
R-free | 0.20580 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.561 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.726 | 1.849 |
High resolution limit [Å] | 1.818 | 1.818 |
Rmerge | 0.113 | 1.479 |
Rmeas | 0.117 | 1.560 |
Rpim | 0.032 | 0.490 |
Number of reflections | 80471 | 3966 |
<I/σ(I)> | 14.8 | 1.4 |
Completeness [%] | 100.0 | |
Redundancy | 13 | |
CC(1/2) | 0.999 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M bicine pH 9.0 1.6 M ammonium sulfate |