6PAJ
Structure of the SrrAB Histidine Kinase DHp-CA domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979,0.9505 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.212, 58.952, 70.860 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 29.920 - 2.000 |
| R-factor | 0.1899 |
| Rwork | 0.187 |
| R-free | 0.24610 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.082 | 69.082 | 2.100 |
| High resolution limit [Å] | 1.990 | 6.290 | 1.990 |
| Rmerge | 0.052 | 0.039 | 0.346 |
| Rmeas | 0.061 | 0.045 | 0.400 |
| Total number of observations | 132910 | 3742 | 19809 |
| Number of reflections | 33780 | 996 | 4923 |
| <I/σ(I)> | 19.9 | 47.2 | 3.7 |
| Completeness [%] | 99.5 | 88.8 | 99.9 |
| Redundancy | 3.9 | 3.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 20% PEG4000, 15% MPD, 0.1 M sodium citrate pH 5.6 |
