6PAE
Dickeya chrysanthemi complex with L-Asp at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.159, 90.400, 127.359 |
| Unit cell angles | 90.00, 91.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1384 |
| Rwork | 0.137 |
| R-free | 0.17570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.660 |
| High resolution limit [Å] | 1.599 | 3.450 | 1.599 |
| Rmerge | 0.055 | 0.035 | 0.335 |
| Number of reflections | 136750 | 15965 | 12933 |
| <I/σ(I)> | 15.1 | ||
| Completeness [%] | 85.8 | 98.6 | 81.6 |
| Redundancy | 2.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | see Miller et al., FEBS Letters, 1993, 328, 275-279 |
