6P8P
Structure of P. aeruginosa ATCC27853 HORMA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 4 |
| Unit cell lengths | 97.525, 97.525, 60.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.525 - 1.635 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.19840 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.530 | 1.660 |
| High resolution limit [Å] | 1.635 | 1.635 |
| Rmerge | 0.093 | 1.786 |
| Rmeas | 0.101 | 1.949 |
| Rpim | 0.039 | 0.768 |
| Number of reflections | 69749 | 3430 |
| <I/σ(I)> | 15 | 1.1 |
| Completeness [%] | 99.7 | 95.4 |
| Redundancy | 6.6 | 6.1 |
| CC(1/2) | 0.999 | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM imidazole pH 8.0, 200 mM CaCl2, and 32% PEG 3350 |
