6P8F
Crystal structure of CDK4 in complex with CyclinD1 and P27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 62.855, 66.628, 184.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.060 - 2.890 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.060 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.180 | 0.990 |
| Rpim | 0.056 | 0.296 |
| Number of reflections | 18044 | 2830 |
| <I/σ(I)> | 14.3 | 2.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 12.9 | 12.8 |
| CC(1/2) | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 100mM Tris (7.0) 17% PEG 3350 100 mM CaCl2 10 mM MgCl2 |
