6P7S

Crystal Structure of the Cedar henipavirus Attachment G Glycoprotein globular domain in complex with the receptor ephrin-B1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9192
Spacegroup name	P 65
Unit cell lengths	207.570, 207.570, 119.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.940 - 3.490
R-factor	0.1976
Rwork	0.196
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.522
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.150	0.082
Rmeas	0.158	0.086
Rpim	0.061	0.026	0.671
Total number of observations	414726
Number of reflections	37299	3818	3691
<I/σ(I)>	5
Completeness [%]	100.0	99.9	100
Redundancy	11.1	10.7	11.1
CC(1/2)		0.997	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	100 mM Tris, pH 8.5, 20% PEG6000