6P7O
Structure of E. coli MS115-1 NucC, Apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.1950 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 208.790, 208.790, 208.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.909 - 1.752 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.19560 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.065 | 1.517 |
| Rmeas | 0.068 | 1.633 |
| Rpim | 0.022 | 0.593 |
| Number of reflections | 39582 | 2097 |
| <I/σ(I)> | 16.5 | 1.2 |
| Completeness [%] | 99.9 | 97.8 |
| Redundancy | 9.3 | 7.2 |
| CC(1/2) | 0.998 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 27% PEG 400, 400 mM MgCl2, and 100 mM HEPES pH 7.5 |
