6P7O

Structure of E. coli MS115-1 NucC, Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.1950
Spacegroup name	F 41 3 2
Unit cell lengths	208.790, 208.790, 208.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.909 - 1.752
R-factor	0.1722
Rwork	0.171
R-free	0.19560
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.065	1.517
Rmeas	0.068	1.633
Rpim	0.022	0.593
Number of reflections	39582	2097
<I/σ(I)>	16.5	1.2
Completeness [%]	99.9	97.8
Redundancy	9.3	7.2
CC(1/2)	0.998	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	27% PEG 400, 400 mM MgCl2, and 100 mM HEPES pH 7.5