6P7J
Crystal structure of Latency Associated Peptide unbound to TGF-beta1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 51.060, 154.900, 62.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.310 - 3.501 |
| R-factor | 0.2897 |
| Rwork | 0.288 |
| R-free | 0.32150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vqp |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.15rc3_3435) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.310 | 36.310 | 3.830 |
| High resolution limit [Å] | 3.500 | 8.570 | 3.500 |
| Rmerge | 0.345 | 0.076 | 1.690 |
| Rmeas | 0.406 | 0.087 | 2.034 |
| Rpim | 0.209 | 0.042 | 1.106 |
| Total number of observations | 12411 | 986 | 2668 |
| Number of reflections | 3332 | 256 | 773 |
| <I/σ(I)> | 3.1 | 6.7 | 1.1 |
| Completeness [%] | 99.6 | 98.3 | 99.5 |
| Redundancy | 3.7 | 3.9 | 3.5 |
| CC(1/2) | 0.899 | 0.988 | 0.467 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.6 | 293 | Reservoir composition: 20% PEG3350, 300 mM NaAc pH 4.6 |
