6P7F

Human ABCC6 NBD2 R1459D mutant in Apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.541780
Spacegroup name	P 41 21 2
Unit cell lengths	99.340, 99.340, 84.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.426 - 2.850
R-factor	0.264349352919
Rwork	0.262
R-free	0.28921
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bzr
RMSD bond length	0.009
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.430	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.189	2.082
Rmeas	0.193	2.119
Rpim	0.037	0.392
Number of reflections	10293	1000
<I/σ(I)>	17.7	1.6
Completeness [%]	99.6	99.7
Redundancy	26.3	28.17
CC(1/2)		0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	0.1 M MES pH 6.5, 1.6 M Ammonium sulfate, 10% dioxane