6P7F
Human ABCC6 NBD2 R1459D mutant in Apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541780 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.340, 99.340, 84.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.426 - 2.850 |
| R-factor | 0.264349352919 |
| Rwork | 0.262 |
| R-free | 0.28921 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bzr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.430 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.189 | 2.082 |
| Rmeas | 0.193 | 2.119 |
| Rpim | 0.037 | 0.392 |
| Number of reflections | 10293 | 1000 |
| <I/σ(I)> | 17.7 | 1.6 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 26.3 | 28.17 |
| CC(1/2) | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.1 M MES pH 6.5, 1.6 M Ammonium sulfate, 10% dioxane |
