6P6E

Structure of Mouse Importin alpha - PAC3 NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.425
Spacegroup name	P 21 21 21
Unit cell lengths	78.490, 90.495, 99.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.669 - 1.990
R-factor	0.1755
Rwork	0.175
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukw
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.669	2.065
High resolution limit [Å]	1.990	1.990
Rmerge	0.118
Rmeas	0.122
Rpim	0.034	0.720
Number of reflections	49103	93402
<I/σ(I)>	18.29
Completeness [%]	99.8	98.78
Redundancy	13
CC(1/2)	1.000	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	Sodium Citrate, DTT, ph 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K