6P5H

Structure of MavC middle insertion domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-27
Detector	RAYONIX MX-300
Wavelength(s)	0.978772
Spacegroup name	P 31
Unit cell lengths	52.027, 52.027, 79.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.229 - 1.530
R-factor	0.2
Rwork	0.199
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc
RMSD bond length	0.006
RMSD bond angle	0.807
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.229	1.585
High resolution limit [Å]	1.530	1.530
Number of reflections	36418	3636
<I/σ(I)>	24.9
Completeness [%]	100.0	100
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	294	2 M ammonium sulfate, 0.2 M lithium sulfate, 0.1 M CAPS: NaOH