6P5B

Crystal Structure of MavC in Complex with Ub-UbE2N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 65
Unit cell lengths	147.844, 147.844, 53.228
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.359 - 2.099
R-factor	0.1969
Rwork	0.195
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc
RMSD bond length	0.002
RMSD bond angle	0.542
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.359	2.174
High resolution limit [Å]	2.099	2.099
Rmerge	0.130	0.136
Number of reflections	38835	3801
<I/σ(I)>	22	2.5
Completeness [%]	99.2	97.89
Redundancy	35.6	10
CC(1/2)		0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0.2 M Na Malonate, 25% PEG 3350, 10 mM NiCl2