6P4Z

Structure of gadolinium-caged cobalt (III) insulin hexamer

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	H 3
格子定数 [Å]	81.400, 81.400, 33.700
格子定数 [度]	90.00, 90.00, 120.00

精密化法

分解能	23.498 - 1.800
R因子	0.1839
Rwork	0.181
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	0.794
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	23.500	1.900
分解能 [Å] (高)	1.800	1.800
独立反射数	7216	1153
<I/σ(I)>	13.5
完全性 [%]	92.9
冗長性	2.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		293	18% (w/v) PEG 12,000 and 0.1 M HEPES pH 7.0