6P4Z
Structure of gadolinium-caged cobalt (III) insulin hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 81.400, 81.400, 33.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.498 - 1.800 |
R-factor | 0.1839 |
Rwork | 0.181 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.794 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.500 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 7216 | 1153 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 92.9 | |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% (w/v) PEG 12,000 and 0.1 M HEPES pH 7.0 |