6P4M
Salmonella typhi PltB Homopentamer with Neu5Ac-alpha-2-3-Gal-beta-1-4-GlcNAc Glycans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.87 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.706, 97.745, 105.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.825 - 1.802 |
R-factor | 0.1695 |
Rwork | 0.169 |
R-free | 0.19320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.204 |
Data reduction software | HKL-2000 (717) |
Data scaling software | HKL-2000 (717) |
Phasing software | PHENIX (1.10.1-2155) |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 66141 | 3078 |
<I/σ(I)> | 21.8 | |
Completeness [%] | 99.3 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG1500, Sodium actetate pH 4.4 |