6P43
Yeast cytochrome c peroxidase in complex with iso-1 cytochrome c (Y48K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97700 |
Spacegroup name | P 1 |
Unit cell lengths | 45.208, 50.890, 86.429 |
Unit cell angles | 105.64, 95.02, 107.31 |
Refinement procedure
Resolution | 23.261 - 1.913 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.076 | 0.036 | 0.592 |
Rmeas | 0.108 | 0.051 | 0.838 |
Rpim | 0.076 | 0.036 | 0.592 |
Total number of observations | 70369 | ||
Number of reflections | 43668 | 1858 | 2246 |
<I/σ(I)> | 14.4 | ||
Completeness [%] | 81.7 | 69.7 | 82.4 |
Redundancy | 1.6 | 1.8 | 1.6 |
CC(1/2) | 0.992 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium acetate, 175 mM NaCl, 5 mM n-octyl-B-D-glucoside, polyethylene glycol 3350 14%-20% |