6P41
Yeast cytochrome c peroxidase (W191Y:L232E) in complex with iso-1 cytochrome c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.803, 112.070, 88.327 |
Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
Resolution | 44.317 - 2.900 |
R-factor | 0.2409 |
Rwork | 0.237 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cih |
RMSD bond length | 0.002 |
RMSD bond angle | 0.542 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
Rmerge | 0.175 | 0.079 | 1.174 |
Rmeas | 0.191 | 0.087 | 1.341 |
Rpim | 0.076 | 0.035 | 0.625 |
Total number of observations | 109662 | ||
Number of reflections | 18648 | 992 | 737 |
<I/σ(I)> | 3.9 | ||
Completeness [%] | 96.2 | 98.9 | 76.6 |
Redundancy | 5.9 | 6.4 | 3.5 |
CC(1/2) | 0.992 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium acetate,175 mM NaCl, 5 mM n-octyl-B-D-glucoside, polyethylene glycol 3350 18%-20% |