6P40

Crystal Structure of Full Length APOBEC3G FKL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	62.067, 86.423, 90.158
Unit cell angles	90.00, 101.38, 90.00

Refinement procedure

Resolution	46.695 - 2.452
R-factor	0.1761
Rwork	0.173
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.470	2.470
Number of reflections	34058	1678
<I/σ(I)>	15.3
Completeness [%]	99.5
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	291	0.1 M MES, pH 6.9 8% PEG 20K