6P3Z

Crystal Structure of Full Length APOBEC3G E/Q (pH 5.2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	67.788, 153.513, 67.800
Unit cell angles	90.00, 114.14, 90.00

Refinement procedure

Resolution	48.171 - 2.844
R-factor	0.2071
Rwork	0.204
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	27992	2278
<I/σ(I)>	8.6
Completeness [%]	94.3
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	291	50 mM MES, pH 5.2 1.7 M lithium sulfate 10 mM MgCl2