6P3Y
Crystal Structure of Full Length APOBEC3G E/Q (pH 7.4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.695, 153.849, 67.728 |
Unit cell angles | 90.00, 116.37, 90.00 |
Refinement procedure
Resolution | 39.169 - 2.570 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.670 |
High resolution limit [Å] | 2.580 | 2.580 |
Number of reflections | 38586 | 3355 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 98.1 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 50 mM Tris, pH 7.4 1.9 M ammonium sulfate 25 mM MgCl2 |