6P3W

Crystal structure of the Cyclin A-CDK2-ORC1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2017-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 62 2 2
Unit cell lengths	186.396, 186.396, 214.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.069 - 2.540
R-factor	0.2001
Rwork	0.198
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1F
RMSD bond length	0.003
RMSD bond angle	0.756
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.070	2.631
High resolution limit [Å]	2.540	2.540
Number of reflections	72513	7055
<I/σ(I)>	9.84
Completeness [%]	99.5
Redundancy	8.1
CC(1/2)	0.997	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.49 M sodium phosphate monobasic monohydrate and 0.91 M potassium phosphate dibasic