6P3W
Crystal structure of the Cyclin A-CDK2-ORC1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-05-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 186.396, 186.396, 214.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.069 - 2.540 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1F |
RMSD bond length | 0.003 |
RMSD bond angle | 0.756 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.070 | 2.631 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 72513 | 7055 |
<I/σ(I)> | 9.84 | |
Completeness [%] | 99.5 | |
Redundancy | 8.1 | |
CC(1/2) | 0.997 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.49 M sodium phosphate monobasic monohydrate and 0.91 M potassium phosphate dibasic |