6P3E
Mobile loops and electrostatic interactions maintain the flexible lambda tail tube
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 5.4 |
| Number of Particle | 7934 |
| Software | EMAN2,MDFF,RELION,EPU,CTFFIND,UCSF Chimera,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
