6P2N
Crystal structure of Paenibacillus graminis GH74 (PgGH74)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.494, 86.214, 130.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.451 - 1.350 |
| R-factor | 0.1499 |
| Rwork | 0.149 |
| R-free | 0.17990 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.289 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.049 | 0.838 |
| Rpim | 0.021 | 0.449 |
| Number of reflections | 136921 | 6842 |
| <I/σ(I)> | 31 | 1.7 |
| Completeness [%] | 96.1 | 96.8 |
| Redundancy | 6.1 | 4.2 |
| CC(1/2) | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 5% (w/v) PEG3350, 0.2 M sodium chloride, 0.1 M sodium citrate pH 5.6, 0.5% (w/v) glycerol |
