6P2K
Crystal structure of AFV00434, an ancestral GH74 enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 173.427, 173.427, 173.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.779 - 2.150 |
| R-factor | 0.1553 |
| Rwork | 0.154 |
| R-free | 0.20410 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.663 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.072 | 0.849 |
| Rpim | 0.032 | 0.441 |
| Number of reflections | 94257 | 4568 |
| <I/σ(I)> | 22.2 | 1.8 |
| Completeness [%] | 99.8 | 97.5 |
| Redundancy | 5.7 | 4.6 |
| CC(1/2) | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 25 mM zinc acetate, 20% (w/v) PEG3350, 1.5 (w/v) MPD |
