6P2A

Chimera of bacteriophage OBP gp146 central spike protein and a T4 gp5 beta-helix fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43
Unit cell lengths	140.013, 140.013, 63.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.502 - 1.900
R-factor	0.1282
Rwork	0.127
R-free	0.15790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jj2
RMSD bond length	0.013
RMSD bond angle	1.142
Data reduction software	XDS (March 30, 2013)
Data scaling software	XDS (March 30, 2013)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.502	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.028	0.175
Rmeas	0.040	0.247
Rpim	0.028	0.175
Number of reflections	193033	19193
<I/σ(I)>	19.65	4.43
Completeness [%]	100.0	99.98
Redundancy	6.8	6.3
CC(1/2)	0.999	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG3350 18-20% 100 mM Ammonium citrate