6P1K
Cryo-EM structure of Escherichia coli sigma70 bound RNAP polymerase holoenzyme
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.05 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
