6P1I
Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 166.417, 169.504, 103.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.927 - 2.740 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6or7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.904 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 38484 | 5873 |
<I/σ(I)> | 12.4 | 2.28 |
Completeness [%] | 99.1 | |
Redundancy | 13.5 | |
CC(1/2) | 1.000 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0 |