6P1B

Transcription antitermination factor Q21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	32.233, 78.905, 68.497
Unit cell angles	90.00, 98.68, 90.00

Refinement procedure

Resolution	39.453 - 1.942
R-factor	0.188
Rwork	0.184
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p1c
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.940	5.260	1.940
Rmerge	0.063	0.048	0.506
Rmeas	0.075	0.058	0.621
Rpim	0.040	0.031	0.354
Total number of observations	81795
Number of reflections	24533	1271	1119
<I/σ(I)>	11.9
Completeness [%]	97.8	98.5	90
Redundancy	3.3	3.3	2.9
CC(1/2)		0.991	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG 3350, 200 mM Ammonium chloride