6P1A

Transcription antitermination factor Q21 in complex with Q21-binding-element DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97
Spacegroup name	P 21 21 2
Unit cell lengths	69.573, 263.224, 56.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.808 - 2.837
R-factor	0.2353
Rwork	0.231
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p1b
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.837	7.730	2.850
Rmerge	0.077	0.034	0.919
Rmeas	0.084	0.038	1.009
Rpim	0.033	0.015	0.409
Number of reflections	25517	1428	1247
<I/σ(I)>	9.9
Completeness [%]	99.6	99.1	99.8
Redundancy	6.3	6.5	5.9
CC(1/2)		0.967	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% PEG 3,350