6P0S

Crystal structure of ternary DNA complex "FX2" containing E. coli Fis and phage lambda Xis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97170
Spacegroup name	P 43 21 2
Unit cell lengths	107.670, 107.670, 150.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.550 - 2.700
R-factor	0.222
Rwork	0.222
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p0t
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (1.3.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.550	87.550	2.770
High resolution limit [Å]	2.700	12.090	2.700
Rmerge	0.116	0.123	0.492
Rmeas	0.121	0.130	0.534
Total number of observations	222119
Number of reflections	23763	343	1528
<I/σ(I)>	12.53	19.34	3.73
Completeness [%]	95.6	100	85.1
Redundancy	9.347	11.534	6.57
CC(1/2)	0.998	0.998	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	15% PEG 4000