6P0J
Crystal structure of GDP-bound human RalA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2019-03-22 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.97625 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.948, 104.809, 55.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.770 - 1.310 |
R-factor | 0.1219 |
Rwork | 0.120 |
R-free | 0.15600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u8z |
RMSD bond length | 0.009 |
RMSD bond angle | 1.075 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.780 | 1.340 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmeas | 0.078 | 1.094 |
Rpim | 0.030 | 0.429 |
Number of reflections | 45413 | 2587 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 99.7 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2 M calcium acetate, pH 5.5, 18-22% PEG3350 |