6P0F
N-terminal domain of Thermococcus Gammatolerans McrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.841, 43.990, 61.963 |
| Unit cell angles | 90.00, 120.28, 90.00 |
Refinement procedure
| Resolution | 53.508 - 1.683 |
| R-factor | 0.1776 |
| Rwork | 0.177 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.199 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.508 | 1.710 |
| High resolution limit [Å] | 1.683 | 1.683 |
| Number of reflections | 33162 | 646 |
| <I/σ(I)> | 35 | 7.2 |
| Completeness [%] | 97.7 | 70.7 |
| Redundancy | 12.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M MES pH 6.5, 3.2 M Ammonium sulfate |
