6OZZ
N terminally deleted GapR crystal structure from C. crescentus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 106.410, 106.410, 139.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.703 - 3.304 |
| R-factor | 0.2871 |
| Rwork | 0.285 |
| R-free | 0.32210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.579 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.116 | 1.250 |
| Number of reflections | 9555 | 49 |
| <I/σ(I)> | 18 | 3.1 |
| Completeness [%] | 76.1 | 5.4 |
| Redundancy | 19.3 | 1.2 |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | 4 % PEG 3350 0.16 M ammonium sulfate 0.6 % 1,2-butanediol |
