6OZB
Crystal structure of the phycoerythrobilin-bound GAF domain from a cyanobacterial phytochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-25 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 78.967, 78.967, 209.299 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.746 - 1.800 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w2z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.078 | 0.038 | 0.629 |
| Rmeas | 0.088 | 0.043 | 0.713 |
| Rpim | 0.039 | 0.019 | 0.329 |
| Total number of observations | 274187 | ||
| Number of reflections | 61064 | 3835 | 2503 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 85.8 | 99.2 | 71.6 |
| Redundancy | 4.5 | 4.9 | 4.2 |
| CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, ammonium acetate, sodium citrate tribasic dihydrate. Crystal was set up under green safety light and grown in dark |
