6OZ2
Crystal structure of the broadly neutralizing antibody N49P6 Fab in complex with HIV-1 Clade A/E strain 93TH057 gp120 core.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.436, 80.815, 195.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.327 - 2.550 |
R-factor | 0.2212 |
Rwork | 0.218 |
R-free | 0.27330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bck |
RMSD bond length | 0.011 |
RMSD bond angle | 1.291 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.276 | 0.727 |
Rpim | 0.166 | 0.556 |
Number of reflections | 31853 | 1524 |
<I/σ(I)> | 6.4 | 1.1 |
Completeness [%] | 92.0 | 89.3 |
Redundancy | 3.1 | 2.2 |
CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 15% PEG 3350 0.1 M Tris-HCl pH 8.0 |