6OYC

Glycosylation Associate Protein (Gap123) complex from Streptococcus agalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	103.009, 168.321, 100.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.272 - 2.351
R-factor	0.1822
Rwork	0.181
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6E2I
RMSD bond length	0.008
RMSD bond angle	0.928
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3051: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.272	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	72701	7084
<I/σ(I)>	28.13
Completeness [%]	99.5
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES pH 7.5, 5% PEG 400, 2M Ammonium sulfate