6OY6

X-ray crystal structure of a bacterial reiterative transcription complex of pyrG promoter at 5 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	186.882, 102.138, 297.364
Unit cell angles	90.00, 98.81, 90.00

Refinement procedure

Resolution	43.666 - 3.096
R-factor	0.2132
Rwork	0.212
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vo8
RMSD bond length	0.012
RMSD bond angle	1.562
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	3.100	3.100
Rpim	0.072	0.695
Number of reflections	88633	2805
<I/σ(I)>	9.64	0.64
Completeness [%]	88.0
Redundancy	2.9	2.3
CC(1/2)		0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000