6OY5
X-ray crystal structure of a bacterial reiterative transcription complex of pyrG promoter at 3 min
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.106, 102.417, 297.237 |
| Unit cell angles | 90.00, 99.00, 90.00 |
Refinement procedure
| Resolution | 43.925 - 3.100 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vo8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.066 | 0.723 |
| Number of reflections | 99590 | 3543 |
| <I/σ(I)> | 11.9 | 0.85 |
| Completeness [%] | 90.0 | 64.4 |
| Redundancy | 3 | 2.5 |
| CC(1/2) | 0.544 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000 |
