6OXA

Dimeric E.coli YoeB bound to Thermus thermophilus 70S pre-cleavage (AAU)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	212.791, 453.079, 608.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.585 - 3.250
R-factor	0.2305
Rwork	0.229
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.585	146.585	3.280
High resolution limit [Å]	3.200	14.320	3.200
Rmerge	0.234	0.023	1.122
Rmeas	0.279	0.027	1.428
Total number of observations	2845730
Number of reflections	883891	10410	51547
<I/σ(I)>	5.87	35.17	0.9
Completeness [%]	92.5	91.3	73.3
Redundancy	3.22	3.337	2.261
CC(1/2)	0.989	0.999	0.297

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1 M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME