6OX0
SETD3 in Complex with an Actin Peptide with Sinefungin Replacing SAH as Cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.265, 175.194, 66.031 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 36.486 - 1.755 |
R-factor | 0.2065 |
Rwork | 0.206 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.466 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.490 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.135 | 1.695 |
Rpim | 0.033 | 0.444 |
Number of reflections | 132568 | 12128 |
<I/σ(I)> | 19.7 | 1.9 |
Completeness [%] | 98.0 | 89.8 |
Redundancy | 15.9 | 12.6 |
CC(1/2) | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |