6OWW

Crystal structure of a Human Cardiac Calsequestrin Filament Complexed with Ytterbium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.3857
Spacegroup name	P 1 21 1
Unit cell lengths	85.832, 86.015, 214.340
Unit cell angles	90.00, 89.91, 90.00

Refinement procedure

Resolution	214.340 - 3.840
R-factor	0.2922
Rwork	0.290
R-free	0.34010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6owv
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	214.340	214.670	3.910
High resolution limit [Å]	3.840	10.420	3.840
Rmeas	0.548	0.126	3.402
Rpim	0.162	0.038	0.990
Total number of observations		16616	17332
Number of reflections	29912	1575	1499
<I/σ(I)>	4.5	19.2	0.8
Completeness [%]	98.9	99.1	97.8
Redundancy	11.2	10.5	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, lithium sulfate