6OWV

Crystal structure of a Human Cardiac Calsequestrin Filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1158
Spacegroup name	P 43 2 2
Unit cell lengths	62.533, 62.533, 213.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.937 - 1.880
R-factor	0.2149
Rwork	0.213
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sji
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.060	71.110	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmeas	0.086	0.048	11.052
Rpim	0.018	0.011	3.234
Total number of observations	799805	44114	20035
Number of reflections	35602	2035	1711
<I/σ(I)>	14.8	47.3	0.4
Completeness [%]	100.0	100	98.6
Redundancy	22.5	21.7	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, lithium sulfate