6OWK
Crystal structure of a Bacillus thuringiensis Cry1B.867 tryptic core variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 106.176, 106.176, 85.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.691 - 2.698 |
| R-factor | 0.2284 |
| Rwork | 0.223 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eb7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.691 | 2.750 |
| High resolution limit [Å] | 2.698 | 2.700 |
| Rmerge | 0.110 | 0.277 |
| Number of reflections | 15172 | 597 |
| <I/σ(I)> | 25.2 | 4.83 |
| Completeness [%] | 93.5 | 79.6 |
| Redundancy | 4.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.3 | 298 | 20% PEG3350, 0.2 M ammonium chloride |
