6OWD
Arginine Containing Reengineered Coiled-Coiled Dimer to Examine the Impact of Proximal Cation Identity on Hydrophobically-Driven Assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.1272 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.730, 31.290, 23.930 |
Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
Resolution | 36.590 - 1.500 |
Rwork | 0.184 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dzm |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.590 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.109 | 1.003 |
Rmeas | 0.118 | 1.086 |
Rpim | 0.046 | 0.411 |
Number of reflections | 8381 | 808 |
<I/σ(I)> | 12.09 | 1.34 |
Completeness [%] | 95.0 | 93.73 |
Redundancy | 6.7 | 7 |
CC(1/2) | 0.997 | 0.424 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | No salt, 0.1 M Bis-Tris pH 5.5, 3.0 M sodium chloride |