6OW7
X-ray Structure of Polypeptide Deformylase with a Piperazic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2005-12-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 43 |
Unit cell lengths | 69.229, 69.229, 91.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.280 - 1.450 |
R-factor | 0.2063 |
Rwork | 0.206 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.808 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.280 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 72134 | 5088 |
<I/σ(I)> | 42.6 | |
Completeness [%] | 94.0 | 66.6 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1 % - 4 % PEG400 45 % - 55% Ammonium Sulfate 0.1 M HEPES |