6OVW

Crystal structure of ornithine carbamoyltransferase from Salmonella enterica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-13
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97927
Spacegroup name	P 43 3 2
Unit cell lengths	139.214, 139.214, 139.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.975 - 1.903
R-factor	0.1678
Rwork	0.167
R-free	0.18620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1duv
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.079	0.048	1.686
Rmeas	0.080	0.049	1.780
Rpim	0.014	0.009	0.560
Total number of observations	1111728
Number of reflections	36844	2070	1803
<I/σ(I)>	7.3
Completeness [%]	100.0	100	99.7
Redundancy	30.2	29.4	9.7
CC(1/2)		0.999	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	2.0 M ammonium dihydrogen phosphate, 0.1 M Tris-Cl